Remove testing code, add garbage collection calls
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@@ -222,9 +222,10 @@ using the (2021 corrected) formula from the original pairSEQ paper. (Howie, et a
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## TODO
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* Try invoking GC at end of workloads to reduce paging to disk
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* ~~Try invoking GC at end of workloads to reduce paging to disk~~ DONE
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* ~~Hold graph data in memory until another graph is read-in?~~
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* No, this won't work, because BiGpairSEQ simulations alter the underlying graph based on filtering constraints. Changes would cascade with multiple experiments.
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* ~~See if there's a reasonable way to reformat Sample Plate files so that wells are columns instead of rows~~ DONE
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* Enable GraphML output in addition to serialized object binaries, for data portability
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* Custom vertex type with attribute for sequence occupancy?
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* Re-implement CDR1 matching method
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@@ -237,10 +238,7 @@ using the (2021 corrected) formula from the original pairSEQ paper. (Howie, et a
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* Implement sample plates with random numbers of T cells per well
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* Possible BiGpairSEQ advantage over pairSEQ: BiGpairSEQ is resilient to variations in well populations; pairSEQ is not.
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* preliminary data suggests that BiGpairSEQ behaves roughly as though the whole plate had whatever the *average* well concentration is, but that's still speculative.
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* See if there's a reasonable way to reformat Sample Plate files so that wells are columns instead of rows
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* Problem is variable number of cells in a well
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* Apache Commons CSV library writes entries a row at a time
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* Can possibly sort the wells by length first, then construct entries
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## CITATIONS
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* Howie, B., Sherwood, A. M., et al. ["High-throughput pairing of T cell receptor alpha and beta sequences."](https://pubmed.ncbi.nlm.nih.gov/26290413/) Sci. Transl. Med. 7, 301ra131 (2015)
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@@ -319,6 +319,7 @@ public class UserInterface {
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assert filename != null;
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CellFileWriter writer = new CellFileWriter(filename, sample);
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writer.writeCellsToFile();
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System.gc();
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}
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// //for calling from command line
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@@ -465,6 +466,7 @@ public class UserInterface {
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System.out.println("Writing Sample Plate to file");
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writer.writePlateFile();
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System.out.println("Sample Plate written to file: " + filename);
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System.gc();
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}
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}
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@@ -496,14 +498,6 @@ public class UserInterface {
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assert plateFile != null;
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PlateFileReader plateReader = new PlateFileReader(plateFile);
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Plate plate = new Plate(plateReader.getFilename(), plateReader.getWells());
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//TAKE THIS OUT
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PlateFileWriter writer = new PlateFileWriter(filename + "_plate", plate);
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System.out.println("Writing Sample Plate to file");
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writer.writePlateFile();
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System.out.println("Sample Plate written to file: " + filename + "_plate");
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//
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if (cellReader.getCells().size() == 0){
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System.out.println("No cell sample found.");
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System.out.println("Returning to main menu.");
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@@ -521,6 +515,7 @@ public class UserInterface {
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System.out.println("File I/O time is not included in results.");
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dataWriter.writeDataToFile();
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System.out.println("Graph and Data file written to: " + filename);
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System.gc();
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}
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}
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@@ -575,6 +570,7 @@ public class UserInterface {
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System.out.println("Writing results to file");
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writer.writeResultsToFile();
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System.out.println("Results written to file: " + filename);
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System.gc();
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}
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///////
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